LB Broth
COMPARISON SUMMARY OF HIETER/BOEKE RECIPE AND BIO 101 PRODUCT

1X LB broth for Bacteria  
Ingredients: Formulation / 5 L
Bacto-tryptone 50g
Yeast extract 25g
Sodium chloride 50g
Distilled water 5000ml
   
BIO-101 LB MEDIUM broth powder  
  Formulation / 5 L
Tryptone 50g
Yeast extract 25g
Sodium chloride 50g
  Total = 125g per packet
Distilled water 5000ml
   
BIO-101 1X LB MEDIUM broth For Bacteria
Ingredients:  
LB-MEDIUM for 5 liter use 1
Distilled water 5000 ml
   
Mix well. Distribute into 100ml or 500ml bottles. Autoclave at 121°C for 20-23 minutes. Mark 1X BIO-101 LB.

 

Last updated by EMC November 25, 1998
 
The Boeke Lab, Johns Hopkins University School of Medicine, 2003