LB Plates
COMPARISON SUMMARY OF HIETER/BOEKE RECIPE AND BIO 101 PRODUCT

L-carb plates for Bacteria  
Ingredients: Formulation / 3 L
Bacto-tryptone 30g
Difco Yeast extract 15g
Sodium chloride 30g
Difco Agar 45g
Distilled water 3000ml
Carbenicillin 3ml
   
BIO-101 LB Agar Medium powder  
  Formulation / 3 L
Tryptone 30g
Yeast extract 15g
Sodium chloride 30g
Difco Agar 45g
  Total = 120g per packet
Distilled water 3000ml
   
BIO-101 1X LB Agar Medium + carb Plates For Bacteria
Ingredients:  
LB-Agar Medium for 3 liter use 1
Distilled water 3000 ml
   
Mix LB-agar medium and distilled water well.

Autoclave, with stir bar in flask, at 121°C for 20-23 minutes.

After autoclaving, stir at room temp. until flask can be handled without gloves. Add Carbenicillin and mix well.

Pour 20ml per plate. (stripe: black)

MARK BOX: BIO 101 LB-CARB PLATES

 

Last updated by EMC November 25, 1998
 
The Boeke Lab, Johns Hopkins University School of Medicine, 2003