YPD + G418
Ingredients:  
BIO 101 Hopkins YPD (YEPD) agar for 3 liters use 1
Distilled Water 3000ml
G418 (200mg/ml) 3ml
(Geneticin)  

 

Directions: Mix Hopkins YPD-agar and distilled water well. Autoclave, with stir bar in flask, at 121°C for 20-23 minutes. After autoclaving, stir at room temp. until flask can be handled without gloves. Add G418. Mix well. Pour 25ml per plate. (stripe: 1 orange) MARK BOX: BIO 101 YPD + G418 (final conc. 200ug/ml

 

Last updated by EMC November 25, 1998
 
The Boeke Lab, Johns Hopkins University School of Medicine, 2003