| Ingredients: | |
| BIO 101 Hopkins YPD (YEPD) agar for 3 liters | use 1 |
| Distilled Water | 3000ml |
| G418 (200mg/ml) | 3ml |
| (Geneticin) |
Directions: Mix Hopkins YPD-agar and distilled water well. Autoclave, with stir bar in flask, at 121°C for 20-23 minutes. After autoclaving, stir at room temp. until flask can be handled without gloves. Add G418. Mix well. Pour 25ml per plate. (stripe: 1 orange) MARK BOX: BIO 101 YPD + G418 (final conc. 200ug/ml