COMPARISON SUMMARY OF HIETER/BOEKE RECIPE AND BIO 101 PRODUCT

YPD plates for yeast  
Ingredients: Formulation / 3 L
Difco Yeast extract 30g
Difco Bacto-peptone 60g
Tryptophan 1.0g
Difco Agar 60g
Distilled water 2500ml
Dextrose 60g
Distilled water 500ml
Mix solutions together after autoclaving
   
BIO-101 YPD (YEPD) agar powder  
  Formulation / 3 L
Yeast extract 30g
Peptone 60g
Dextrose 60g
Tryptophan .96g
Agar 51g
  =201.96g per packet
Distilled water 3000ml
   
BIO-101 Boeke Media YPD (YEPD) plates For yeast
Ingredients:  
Hopkins YPD (YEPD) agar for 3 liters use 1
Distilled water 3000 ml
   
Mix well. Autoclave at 121°C for 20-23 minutes. Cool to 50°C. Mix well. Pour 25ml per plate. (stripe: green) MARK BOX: BIO 101 YPD PLATES

 

Last updated by EMC November 25, 1998
 
The Boeke Lab, Johns Hopkins University School of Medicine, 2003