| Ingredients | for yeast |
| #1: | |
| YNB w/o a.a.'s, amm. sulf. | 8.5 g |
| Ammonium sulfate | 25 g |
| -arginine drop out mix | 10 g |
| Distilled water | 2000ml |
| #2: | |
| Dextrose | 100 g |
| Distilled water | 500 ml |
| #3: | |
| Difco agar | 100 g |
| Distilled water | 2500 ml |
Directions: Autoclave #1,2,3, separately (#3 with stir bar). After autoclaving, combine #1,2 with 3 and mix well. Stir at room temp. until flask can be handled without gloves. Add 15ml of a 20mg/ml canavanine stock soln. (Final conc. of canavanine = 60ug/L.). Mix well. Pour 25ml per plate. Canavanine sulfate is typically prepared as a filter-sterilized (0.2um filter) 20mg/ml stock solution in distilled water. It is stored at 4°C