This document is best viewed in landscape orientation with a 7 point non-proportional font and 0.5 inch margins.

The general structure of these sequences is Target DNA - extranucs (if any) - L1 cDNA.

ks500= Bluescript KS- origin of replication (L1 target DNA).

Microhomology often exists between the L1 polyT tail and the T rich target sequence (and occasionally between internally initiated L1 cDNA and the target). For the purpose of this supplementary data, this has ambiguous sequence has been assigned to the L1 cDNA.

Non-templated nucleotides are typically separated from the target and L1 cDNA by spaces. In several instances (RNAs #2: 28, 35; #4: 19/T7, 28Bq, 25Bq; and #7: 4, 15), other segments of KS-DNA are found between the target DNA and L1 cDNA. These clones were not excluded from the analysis, but may be the product of an abberant mechanism.

For clarity, the internal polyT heterogeneity with L1 cDNA #4 is not shown in Figure #3c.

The RNAs #3 used in WT, EN-,and "QF" TPRT reactions were produced in three separate T7 transcription reactions and have varying amounts of T7 polyA slippage. WT "QF" insertions are therefore not shown in Figure #3c.

The data set is non-redundant. This may have resulted in the exclusion of a few identical yet independent insertions.

Download the Microsoft Word document here.