Our lab has developed vectors for C. elegans transgenesis.
These vectors are for germline expression only. They use
sequences from the pie-1 gene (promoter, intron and 3’ sequences)
to drive expression of ORFs (or GFP-ORF fusions) in the maternal
germline. The pie-1 vectors are especially well suited for
maternal expression of transgenic proteins in oocytes and
early embryos.
THIRD GENERATION SEYDOUX LAB VECTOR KIT
The purpose of kit is to create transgenes that can be used
to transform unc-119 worms by bombardment. The kit is modular
allowing the user to choose promoter, ORF, and 3’ sequences
and thus is suitable for expression in any tissue.
NEWS!!! The third generation Seydoux Lab Vector kit
is now available from Addgene |
