Department of Neuroscience / HHMI
Johns Hopkins University School of Medicine
Email: dlin13@jhu.edu
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Department of Neuroscience / HHMI Johns Hopkins University School of Medicine Email: dlin13@jhu.edu |
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Two photon imaging allow us to image deep into scattering tissues such as intact brains. To examine how AMPAR trafficking might affect neuronal function in vivo, I have recently build a custom two-photon laser scanning system for in vivo imaging. The microscope is based on Sutter MOM. The scanning galvo and servo driver are from Cambridge Technology, data acquisition is through a National Instrument DAQ board, and the detectors are GaAsP PMTs from Hamamatsu. ScanImage is used to run the imaging system. The in vivo system shares a 6x8 table with the two-photon uncaging system. A 50/50 beam splitter was used to split the imaging laser beam. For laser safety reasons, during normal operation, all the laser beam is enclosed with custom build safety covers. A custom-build passive beam splitter and a pre-chirping unit will be incorporated in the routing optics to improve the performance of this system in the near future. The setup:
The setup: (beam routing on the tables for both systems)
What can we do: in vivo imaging of neuronal morphology and dynamics (Neurons in Barrel cortex in vivo, 780 um in Z direction)
(Cerebellar Purkinje neuron in vivo)
Intrinsic optical signalin (IOS) imaging allows one to functional mapping of brain regions in response to stimulation, example below is IOS imaging of mouse barrel cortex in resopnse to whisker stimulation:
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