The Synthesis and Sequencing Facility uses one 394 DNA synthesizer and one ABI 380B DNA Synthesizer. We offer synthesis in four scales at the following rates:
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Turnaround time for Oligos is usually 1-2 days. Oligo orders delivered with a template will be cycle sequenced and subsequently loaded on our automated DNA sequencer as soon as the oligo is ready.
Click here for a detailed explanation of the automated synthesis process as performed on the ABI 380B DNA Synthesizer.
Reverse phase HPLC purification is available for a charge of $20.00 per oligo. Purification for many applications is not necessary, including PCR primers, manual and automated sequencing. A crude 40 nmol synthesis will yield 4-12 uM of primer (20-mer) if diluted in 1 ml of water. This is enough for at least 1000 automated sequencing reactions. Your sample may need more than one purification step (no extra charge), depending on the length and if the oligos have been modified with specialty items.
Oligos are accompanied by an information sheet which gives melting temperature and concentration information. Click here for other calculations and useful info and the Oligo Calculator to assist you in working with your oligonucleotides.
We can custom design oligos to be used for automated DNA sequencing at no additional charge.
There are many different 5' or 3' modifiers, labeling reactions,
and monomer replacements that can be performed on the ABI 380B DNA
synthesisers. Click here to get information on the most common.
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