Microscopic mechanism of DNA damage searching by hOGG1. Nucleic Acids Res. 2014; 42, 9295-9303
GTP activator and dNTP substrates of HIV-1 restriction factor SAMHD1 generate a long-lived activated state. PNAS.
2014; 111, 1843-1851
Uracil DNA glycosylase initiates degradation of HIV-1 cDNA containing misincorporated dUTP and prevents viral integration. PNAS. 2013; 110, 448-457
Timing facilitated site transfer of an enzyme on DNA. Nature Chem Biol. 2012; 8, 205–210
Detection of damaged DNA bases by DNA glycosylase enzymes. Biochemistry. 2010; 49, 4957-4967
Indirect Detection of Labile Solute Proton Spectra via the Water Signal Using Frequency-Labeled Exchange (FLEX) Transfer. JACS. 2010; 132, 1813-1815.
Impact of linker strain and flexibility in the design of a fragment-based inhibitor. Nature Chem Biol. 2009; 5, 407-413.
Enzymatic Capture of an Extrahelical Thymine in the Search for Uracil in DNA. Nature. 2007; 449, 433-438.

Welcome to the Stivers Lab Webpage

My laboratory is broadly interested in the biology of the RNA base uracil when it is present in DNA. Our work involves structural and biophysical studies of uracil recognition by DNA repair enzymes, the central role of uracil in adapative and innate immunity, and the function of uracil in antifolate and fluoropyrimidine chemotherapy. Accordingly, we use a wide breadth of structural, chemical, genetic and biophysical approaches that provide a fundamental understanding of molecular function. Our long-range goal is to use this understanding to design novel small molecules that alter biological pathways within a cellular environment. One approach we are developing is the high-throughput synthesis and screening of small molecule libraries directed at important targets in cancer and HIV-1 pathogenesis.