Arun Sharma Years at JHU: 3.5 years (Class of 2003) Major: Biology / History of Science Awards and honors: a) Provost’s Undergraduate Research Award b) Dean's List ('99 - '03) c) Editor-in-Chief of Hopkins Undergraduate Research Journal Years worked in Hubbard lab: Fall 2000 - Spring 2003 Techniques learned/used: Light microscopic localization of polarity proteins in WIF-B cells, Confocal Microscopy, Purification of recombinant proteins from bacteria, western blotting and Tissue Culture. Project involved in: Characterization of Complex I Components (aPKC, PAR3, and PAR6) in Hepatic PolarityE-mail address: arunsharma@jhu.edu Future: University of Pittsburgh School of Medicine, 2003-2007
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Image by Arun Sharma
aPKC is found at the cell apex in early WIF -B cells ( A , A') and at the TJ and apical PM in mature WIF-B cells (B)
(A)WIF -B cells were fixed and stained with anti-aPKC (C-20) and anti-ZO-1( A and B). Images A and A' are focused at the cell apex of early WIF -B cells , arrow.(B) A merged stack of confocal images shows co-localization of aPKC (green) with ZO-1 (red) at the TJ , which forms a belt (arrow) around apical cysts. The apical PM is stained with anti-aPKC. Cytosolic staining is not visible.