The Boeke Lab - The Met15 Resource

The Met15 Resource

Introduction	Plasmid Map	Update
Designer Deletion Strains		Publications

Map of the MET15 pRS plasmids

from Brachmann et al. (1998)

Sequence of the pRS4X1 series and other MET15 related plasmids

pRS401- (ATCC#87473) (Genbank#U93714) - integrating, MET15; see Brachmann et al. (1998)
pRS411- (ATCC#87474) (Genbank#U93715) - CEN/ARS, MET15; see Brachmann et al. (1998)
pRS421- (ATCC#87475) (Genbank#U93716) - 2 micron, MET15; see Brachmann et al. (1998)
pGC3- (ATCC#87440) - MET15 SpeI-SpeI genomic clone; see Cost and Boeke (1996)
pAD4- (ATCC#87470) - MET15 deleter plasmid; see Brachmann et al. (1998)

Sequence of Related pRS series plasmids

pRS400- (Genbank#U93713) -integrating kanMX4 marker; see Brachmann et al. (1998)
pRS402- (ATCC#87477) (Genbank#U93717) -integrating, ADE2 marker; see Brachmann et al. (1998)
pRS412- (ATCC#87478) (Genbank#U93718) - CEN/ARS, ADE2 marker; see Brachmann et al. (1998)
pRS422- (ATCC#87479) (Genbank#U93719) - 2 micron, ADE2 marker; see Brachmann et al. (1998)
pGC25- centromeric, no marker; see Brachmann et al.
pGC26- 2 micron, no marker; see Brachmann et al.

pRS plasmids (of published sequence) also exist in integrating, CEN/ARS, and 2micron form with the HIS3, TRP1, LEU2, URA3, and LYS2 (except integrating) markers.

Gregory J. Cost 1 February1999

All information contained herein is believed to be true to the best of my knowledge; no warranty is expressed or implied... I try my best- feedback (especially positive) is welcome.

The Boeke Lab, Johns Hopkins University School of Medicine, 2003