The Met15 Resource
Map of the MET15 pRS plasmids
from Brachmann et al. (1998)
Sequence of the pRS4X1 series and other MET15 related
plasmids
Sequence of Related pRS series plasmids
- pRS400- (Genbank#U93713)
-integrating kanMX4 marker; see Brachmann et al. (1998)
- pRS402- (ATCC#87477)
(Genbank#U93717)
-integrating, ADE2 marker; see Brachmann et al. (1998)
- pRS412- (ATCC#87478)
(Genbank#U93718)
- CEN/ARS, ADE2 marker; see Brachmann et al. (1998)
- pRS422- (ATCC#87479)
(Genbank#U93719)
- 2 micron, ADE2 marker; see Brachmann et al. (1998)
- pGC25- centromeric, no marker; see Brachmann et
al.
- pGC26- 2 micron, no marker; see Brachmann et al.
pRS plasmids (of published sequence) also exist in integrating,
CEN/ARS, and 2micron form with the HIS3,
TRP1, LEU2, URA3, and LYS2 (except integrating) markers.
Gregory J. Cost 1 February1999
All information contained herein is believed to be true to the best
of my knowledge; no warranty is expressed or implied... I try my best-
feedback (especially
positive) is welcome.
The Boeke Lab, Johns Hopkins University School of Medicine, 2003