| Publications |
Culture Medium: Current | Culture Medium: Old
| Culture Conditions | Recipes: Current |
Recipes: Old | Cell Thawing |
Cell
Characteristics | Subculture: Current | Subculture: Old |
Quality Control | Cell IMF Protocols
| Preservation (Cell Freezing) | DNA Delivery
|
Culture Conditions:
- 7% CO2 atmosphere
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37° C temperature
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Medium is renewed (changed) three times a week (ex. For 10 cm dish: 15 ml on Mon. and Weds., 20 ml on Fri).
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Cells are grown on tissue culture dishes (e.g. Falcon #3003 10 cm tissue culture dish (TCD)), and glass cover slips.
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A confluent (1.5 x 105 cells/cm2) container is split 1:4-16 (see subculture protocol).
- If cells are being grown for routine maintenance, the cells should be passed approximately every 10 days, when the cells have been sown at a density of 1-2 x
105/10 cm dish.