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Cell Characteristics

• Generation time is 1 generation/~72 hours.
• Cloning efficiency is 80-90%.
• Cell pellet should be light brown in color.
• The cells have 1 to 2 nucleoli.
• During mitosis the cells are attached.
• The cells form bile canaliculi (BC), phase lucent structures.
• When cells are confluent, they are viable in this stage for 1 week.
• Cells are very similar to WIF-12-1 cells, except they do not go into the crisis stage.
• The cells grow better and produce more BC when cells are in high density, >2.8 x 10⁴ cell/cm² (1.5 x 10⁶ cell/10cm dish, for experimental set up). When the cells are confluent they exhibit many BC.
• At confluency 90% of the cells exhibit BC.
• For additional characteristics see the publications,
• Cells do begin to show signs of apoptosis after 7 to 10 days in culture. This can increase with further time in the culture during a given passage. Cellular debris is clearly visible, cells can be rinsed (F12 without serum or a HANKs buffer are fine), then fed to remove debris