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Recipes (Current):

1. 1000x HAT Stock

Materials:

• Hypoxanthine, Anhydrous M.W. 136.1, Sigma Cat. #H-9636
• Aminopterin, Anhydrous M.W. 440.4, Sigma Cat. #A-3411
• Thymidine, Anhydrous M.W. 242.2, Sigma Cat. #T-1895

Protocol:

1. Add to 136.12 mg Hypoxanthine 0.6 ml 1 N NaOH and dissolve.
2. Add 1.76 mg Aminopterin to a couple drops of 1 N NaOH and dissolve.
3. Combine #1, #2 and 38.76 mg Thymidine with 100 ml with DDH₂O.
4. Sterile filter (0.22 m m).
5. Make 5ml aliquots, incubate overnight at 4° C and then store at -20° C.

2. 500x Amphotericin B (Fungizone) Stock (250 m g/ml)

1. Amphotericin B (Fungizone), Sigma Cat. #A-2942.