| Publications | Culture Medium: Current | Culture Medium: Old | Culture Conditions | Recipes: Current | Recipes: Old | Cell Thawing | Cell Characteristics | Subculture: Current | Subculture: Old | Quality Control | Cell IMF Protocols | Preservation (Cell Freezing) | DNA Delivery |

Recipes (Current):

  1. 1000x HAT Stock

Materials:

Protocol:

  1. Add to 136.12 mg Hypoxanthine 0.6 ml 1 N NaOH and dissolve.
  2. Add 1.76 mg Aminopterin to a couple drops of 1 N NaOH and dissolve.
  3. Combine #1, #2 and 38.76 mg Thymidine with 100 ml with DDH2O.
  4. Sterile filter (0.22 m m).
  5. Make 5ml aliquots, incubate overnight at 4 C and then store at -20 C.
  1. 500x Amphotericin B (Fungizone) Stock (250 m g/ml)
  1. Amphotericin B (Fungizone), Sigma Cat. #A-2942.