| Publications |
Culture Medium: Current | Culture Medium: Old
| Culture Conditions | Recipes: Current |
Recipes: Old | Cell Thawing |
Characteristics | Subculture: Current | Subculture: Old |
Quality Control | Cell IMF Protocols
| Preservation (Cell Freezing) | DNA Delivery
Aliquots (vials are thawed rapidly and reseeded within recommended sowing range.
*When thawing, most cell death occurs between –50° C and 0°
- Always check cells the following day for healthy, viable cells and freedom from contamination.
Protocol for 1x106 cells/vial:
- Remove vial from liquid nitrogen and immerse immediately into 37° C water bath until the medium is liquid, (approximately 2 minutes), and constantly agitate.
- Tighten vial cap, ethanol vial thoroughly on outside. Add 0.5 ml of medium into the vial, drop by drop. Add cell suspension into 8 ml of fresh medium in a 15 ml conical tube (9 ml total).
- Plate cells in two 10 cm dishes, 3 ml and 6 ml, respectively.
- Place in the incubator, and once the cells have attached, renew with fresh medium (do this no later than 24 hours). Observe cells daily for growth and freedom from contamination.